Table_TAILPCR

Table 1 - T-DNA insertions in C24 (RD29A::LUC)

Mutant # Category Left border Flanking DNA and/or Protein Description

1043-1 los cold/ABA Yes blast hit with putative sugar transporter

106-36 cos Yes match to cryptochrome2 apoprotein(CRY2), photolysase (PHH1)

1129-2 hos all Yes putative retrotransposon

120-4 hos cold/ABA; los salt? No before unknown protein

1271-4 cos/hos all Yes similar to Arabidopsis predicted protein

1271-6 not mutant Yes between putative proteins

1294-2 hos cold Yes? unknown protein

1370-1 hos cold Yes? between MADS-box protein-like and WD40 repeat protein-like

1408-2 los cold/ABA Yes? between receptor-like protein kinase and hypothetical gene

1419-1 hos cold Yes? 1.2kb upstream of putative ABC transporter

1422-1 los cold/ABA Yes? putative CAP-binding protein

1422-2 los cold/ABA Yes? putative CAP-binding protein

1431-2 los cold/ABA Yes? unknown protein (before protein with weak sim. to 1-asparaginase)

1437-1 los cold/ABA Yes? phenylalanyl-tRNA synthetase-like protein

1468-3 los cold/ABA Yes? ATN1-like protein kinase

1471-4 los cold/ABA Yes? between hypothetical protein and putative Pectinesterase

1483-3 los cold/ABA Yes? between receptor-like protein kinase and hypothetical gene

1486-3 los cold/ABA Yes? putative Nematode-resistance protein

1494-2 hos ABA Yes? unknown protein

1623-2 hos cold/ABA Yes? RNA polymerase III subunit-like protein

1628-1 hos cold/ABA Yes? hypothetical protein(after put. CONSTANS-like B-box zinc finger)

1629-1 hos cold Yes? putative eukaryote initiation factor 4, eIF4

166-1 hos cold No before unknown protein BAB10547.1

181-6 los all No between unknown and protein sim. to polypeptide deformylase

1927-3 los all No in repeat reg. between putative embryo-abundant protein & hypoth. protein

1928-3 los all Yes between put. protein and put. Protein containing EF-hand Ca-binding dom.

1928-3 los all Yes between put. receptor kinase and hypothetical protein

1928-4 hos all Yes in front of protein with sim. to NADP specific Isocitrate dehydrogenase

1981-2 los all Yes between put. sucrose-phosphate synthase and put. growth promoter

1981-2 los all No? putative ATP-dependent RNA helicase A

1997-1 los ABA Yes similar to alanine aminotransferase AAB01685.1

2011-1 not determined Yes match to cryptochrome2 apoprotein(CRY2), photolysase (PHH1)

2017-2 not determined Yes between hypotetical protein and gene sim. to Pv42P

2080-1 hos all Yes between putative snRNP splicing factor and put. selenium binding protein

2080-1 hos all Yes unknown protein (blast hit with putative cytoskeletal protein)

2080-2 los all Yes? between putative protein and adenosine-5-phosphosulfate kinase

2082-2 los all No the end of BAC; repeat region containing centromeric repeat

2082-2 los all Yes? unknown protein (blast hit with putative cytoskeletal protein)

2109-2 hos ABA?/ABA24 Yes between unknown protein and glycosylation enzyme-like protein

2109-5 los all No? 26s ribosomal RNA

2110-1 los cold/ABA?/salt No between hypothetical protein and Mei2-like protein

2110-1 los cold/ABA?/salt No MADS box transcription factor-like

2112-1 los all Yes between put. protein and E2 ubiquitin-conjugating-like enzyme Ahus5

2112-1 los all Yes? putative protein similar to MEL-26

2116-2 hos cold Yes between unknown proteins

2118-2 los ABA? No between put. proteins--one sim. to 70K peroxisomal membrane protein

2129-2 hos cold/ABA Yes between 40S ribosomal protein and protein kinase

2149-1 los ABA No between unkn. prot. and prot. sim. to Zn-finger DNA binding protein KIN17

2149-1 los ABA Yes between unknown proteins

2196-2 los cold??/ABA?? Yes between protein phosphatase 2C-like protein and hypothetical protein

2230-4 hos cold?/ABA No putative protein kinase

2282-1 los ABA? No between HSF30-like homologs

2298-5 los ABA Yes? between teosinte branched1 - like protein and hypothetical protein

2298-5 los ABA No? homology to fragments of reverse transcriptase

2300-2 los cold/ABA No myo-inositol-1-phosphate synthase

2529-1 hos ABA?/salt? No before unknown protein

2576-1 los cold/ABA Yes Putative protein after putative Ap2 domain protein

2620-1 cos?/hos all No 3(2),5-bisphosphate nucleotidase

2620-2 cos?/hos all No between unknown protein and 3(2),5-bisphosphate nucleotidase

2620-2 cos?/hos all No DNA polymerase III catalytic subunit

2638-1 los cold/ABA Yes blast hit with probable membrane protein YOR054c; contains F box

2640-4 cos?/hos salt Yes between putative protein phosphatase 2C and repeat region

2645-5 cos?/hos all No between RXF26-like protein and DNA binding protein

2645-5 cos?/hos all Yes Dehydrin-likeprotein (Xero1)

2690-6 hos ABA/ABA24/salt Yes? between hypothetical protein and putative protein

2711-2 los all Yes 1.2kb away from receptor-like protein

2711-2 los all Yes between hypoth. prot. & prot. sim. to cytochrome C oxidase polypeptide I

2767-2 hos cold?/ABA? No? about 500 bp away from 5'-beta1-3 glucanase-like protein

2782-2 los cold?/ABA Yes Hypothetical protein

73-6 hos ABA/salt No unknown protein(between amylogenin and prot. sim. to auxin transport prot.)

905-5 los cold/ABA Yes? putative Cytochrome P450 monooxygenase

905-7 hos cold Yes? between cinnamylalcohol dehydrogenase 2 and hypothetical protein

2182 cos Yes disease resistant protein-like

HA324-1 hos ABA/ABA24 Yes between unkn. prot. & indole-3-acetate beta-glucosyltransferase-like prot.

LA41 los all Yes? transposon protein-like

LA41 los all Yes putataive TNP2-like transposon protein

Ta33 los all Yes between putative phospatase 2A inhibitor and hypothetical protein

Ta4 los all No between unknown proteins

Tb30 hos cold Yes after putative DNA binding protein

Tb53 los all No unknown protein (between disease resistance protein and unknown protein)

Tc14 los all Yes 700bp downstream of putative endochitinase

Tc18 hos all Yes heat-shock protein

Tc19 los all No rRNA repeat

Tc26 los all Yes between transcription regulator-like protein and unknown protein

Tc28 hos ABA Yes between hypothetical protein and auxin-responsive GH3-like protein

Tc28 hos ABA Yes between protein similar to cellulase and unknown protein

Tc29 hos ABA Yes between hypothetical protein and putative cyclin

Tc30 los all Yes Argonaute (AGO1)-like protein

Tc30 los all Yes between unknown proteins

Tc32 los all Yes Argonaute (AGO1)-like protein

Tc35 los all Yes between unkn proteins(blast hits with tyramine hydroxycinnamoyltransferase)

Tc36 los all Yes hypothetical protein (before putative 50s ribosomal protein L13)

Tc36 los all Yes hypothetical protein (before putative 50s ribosomal protein L13)

Tc36 los all Yes hypothetical protein (before putative 50s ribosomal protein L13)

Tc65 los all Yes putative protein containing prenyl group binding site (CAAXbox)

Td18 hos cold Yes hypothetical protein

Td45 hos all Yes 200bp upstream of cytosolic cyclophilin (ROC3)

Te3 los all Yes hypothetical protein similar to HETI protein

Tf114 hos cold Yes between alpha-amylase-like protein and MtN3-like protein

Tf120 hos ABA Yes between unknown proteins

YES? - indicates that sequences did not extend to the left border but that its presence could be deduced from the length of the DNA fragment.

los - low expression of osmotically responsive genes to ….

hos - high expression of osmotically responsive genes to ….

Mutant #	Category	Left border	Flanking DNA and/or Protein Description
1043-1	los cold/ABA	Yes	blast hit with putative sugar transporter
106-36	cos	Yes	match to cryptochrome2 apoprotein(CRY2), photolysase (PHH1)
1129-2	hos all	Yes	putative retrotransposon
120-4	hos cold/ABA; los salt?	No	before unknown protein
1271-4	cos/hos all	Yes	similar to Arabidopsis predicted protein
1271-6	not mutant	Yes	between putative proteins
1294-2	hos cold	Yes?	unknown protein
1370-1	hos cold	Yes?	between MADS-box protein-like and WD40 repeat protein-like
1408-2	los cold/ABA	Yes?	between receptor-like protein kinase and hypothetical gene
1419-1	hos cold	Yes?	1.2kb upstream of putative ABC transporter
1422-1	los cold/ABA	Yes?	putative CAP-binding protein
1422-2	los cold/ABA	Yes?	putative CAP-binding protein
1431-2	los cold/ABA	Yes?	unknown protein (before protein with weak sim. to 1-asparaginase)
1437-1	los cold/ABA	Yes?	phenylalanyl-tRNA synthetase-like protein
1468-3	los cold/ABA	Yes?	ATN1-like protein kinase
1471-4	los cold/ABA	Yes?	between hypothetical protein and putative Pectinesterase
1483-3	los cold/ABA	Yes?	between receptor-like protein kinase and hypothetical gene
1486-3	los cold/ABA	Yes?	putative Nematode-resistance protein
1494-2	hos ABA	Yes?	unknown protein
1623-2	hos cold/ABA	Yes?	RNA polymerase III subunit-like protein
1628-1	hos cold/ABA	Yes?	hypothetical protein(after put. CONSTANS-like B-box zinc finger)
1629-1	hos cold	Yes?	putative eukaryote initiation factor 4, eIF4
166-1	hos cold	No	before unknown protein BAB10547.1
181-6	los all	No	between unknown and protein sim. to polypeptide deformylase
1927-3	los all	No	in repeat reg. between putative embryo-abundant protein & hypoth. protein
1928-3	los all	Yes	between put. protein and put. Protein containing EF-hand Ca-binding dom.
1928-3	los all	Yes	between put. receptor kinase and hypothetical protein
1928-4	hos all	Yes	in front of protein with sim. to NADP specific Isocitrate dehydrogenase
1981-2	los all	Yes	between put. sucrose-phosphate synthase and put. growth promoter
1981-2	los all	No?	putative ATP-dependent RNA helicase A
1997-1	los ABA	Yes	similar to alanine aminotransferase AAB01685.1
2011-1	not determined	Yes	match to cryptochrome2 apoprotein(CRY2), photolysase (PHH1)
2017-2	not determined	Yes	between hypotetical protein and gene sim. to Pv42P
2080-1	hos all	Yes	between putative snRNP splicing factor and put. selenium binding protein
2080-1	hos all	Yes	unknown protein (blast hit with putative cytoskeletal protein)
2080-2	los all	Yes?	between putative protein and adenosine-5-phosphosulfate kinase
2082-2	los all	No	the end of BAC; repeat region containing centromeric repeat
2082-2	los all	Yes?	unknown protein (blast hit with putative cytoskeletal protein)
2109-2	hos ABA?/ABA24	Yes	between unknown protein and glycosylation enzyme-like protein
2109-5	los all	No?	26s ribosomal RNA
2110-1	los cold/ABA?/salt	No	between hypothetical protein and Mei2-like protein
2110-1	los cold/ABA?/salt	No	MADS box transcription factor-like
2112-1	los all	Yes	between put. protein and E2 ubiquitin-conjugating-like enzyme Ahus5
2112-1	los all	Yes?	putative protein similar to MEL-26
2116-2	hos cold	Yes	between unknown proteins
2118-2	los ABA?	No	between put. proteins--one sim. to 70K peroxisomal membrane protein
2129-2	hos cold/ABA	Yes	between 40S ribosomal protein and protein kinase
2149-1	los ABA	No	between unkn. prot. and prot. sim. to Zn-finger DNA binding protein KIN17
2149-1	los ABA	Yes	between unknown proteins
2196-2	los cold??/ABA??	Yes	between protein phosphatase 2C-like protein and hypothetical protein
2230-4	hos cold?/ABA	No	putative protein kinase
2282-1	los ABA?	No	between HSF30-like homologs
2298-5	los ABA	Yes?	between teosinte branched1 - like protein and hypothetical protein
2298-5	los ABA	No?	homology to fragments of reverse transcriptase
2300-2	los cold/ABA	No	myo-inositol-1-phosphate synthase
2529-1	hos ABA?/salt?	No	before unknown protein
2576-1	los cold/ABA	Yes	Putative protein after putative Ap2 domain protein
2620-1	cos?/hos all	No	3(2),5-bisphosphate nucleotidase
2620-2	cos?/hos all	No	between unknown protein and 3(2),5-bisphosphate nucleotidase
2620-2	cos?/hos all	No	DNA polymerase III catalytic subunit
2638-1	los cold/ABA	Yes	blast hit with probable membrane protein YOR054c; contains F box
2640-4	cos?/hos salt	Yes	between putative protein phosphatase 2C and repeat region
2645-5	cos?/hos all	No	between RXF26-like protein and DNA binding protein
2645-5	cos?/hos all	Yes	Dehydrin-likeprotein (Xero1)
2690-6	hos ABA/ABA24/salt	Yes?	between hypothetical protein and putative protein
2711-2	los all	Yes	1.2kb away from receptor-like protein
2711-2	los all	Yes	between hypoth. prot. & prot. sim. to cytochrome C oxidase polypeptide I
2767-2	hos cold?/ABA?	No?	about 500 bp away from 5'-beta1-3 glucanase-like protein
2782-2	los cold?/ABA	Yes	Hypothetical protein
73-6	hos ABA/salt	No	unknown protein(between amylogenin and prot. sim. to auxin transport prot.)
905-5	los cold/ABA	Yes?	putative Cytochrome P450 monooxygenase
905-7	hos cold	Yes?	between cinnamylalcohol dehydrogenase 2 and hypothetical protein
2182	cos	Yes	disease resistant protein-like
HA324-1	hos ABA/ABA24	Yes	between unkn. prot. & indole-3-acetate beta-glucosyltransferase-like prot.
LA41	los all	Yes?	transposon protein-like
LA41	los all	Yes	putataive TNP2-like transposon protein
Ta33	los all	Yes	between putative phospatase 2A inhibitor and hypothetical protein
Ta4	los all	No	between unknown proteins
Tb30	hos cold	Yes	after putative DNA binding protein
Tb53	los all	No	unknown protein (between disease resistance protein and unknown protein)
Tc14	los all	Yes	700bp downstream of putative endochitinase
Tc18	hos all	Yes	heat-shock protein
Tc19	los all	No	rRNA repeat
Tc26	los all	Yes	between transcription regulator-like protein and unknown protein
Tc28	hos ABA	Yes	between hypothetical protein and auxin-responsive GH3-like protein
Tc28	hos ABA	Yes	between protein similar to cellulase and unknown protein
Tc29	hos ABA	Yes	between hypothetical protein and putative cyclin
Tc30	los all	Yes	Argonaute (AGO1)-like protein
Tc30	los all	Yes	between unknown proteins
Tc32	los all	Yes	Argonaute (AGO1)-like protein
Tc35	los all	Yes	between unkn proteins(blast hits with tyramine hydroxycinnamoyltransferase)
Tc36	los all	Yes	hypothetical protein (before putative 50s ribosomal protein L13)
Tc36	los all	Yes	hypothetical protein (before putative 50s ribosomal protein L13)
Tc36	los all	Yes	hypothetical protein (before putative 50s ribosomal protein L13)
Tc65	los all	Yes	putative protein containing prenyl group binding site (CAAXbox)
Td18	hos cold	Yes	hypothetical protein
Td45	hos all	Yes	200bp upstream of cytosolic cyclophilin (ROC3)
Te3	los all	Yes	hypothetical protein similar to HETI protein
Tf114	hos cold	Yes	between alpha-amylase-like protein and MtN3-like protein
Tf120	hos ABA	Yes	between unknown proteins
YES? - indicates that sequences did not extend to the left border but that its presence could be deduced from the length of the DNA fragment.
los - low expression of osmotically responsive genes to ….
hos - high expression of osmotically responsive genes to ….